Application

Flow Cytometry: ≤ 1 µg per 10E6 cells

ELISA

Immunohistochemistry: 10µg/mL

Blocking of Ligand Binding

Western Blotting

Optimal working dilutions must be determined by the end user.

Research CategorySignaling

Detect CD140a using this Anti-CD140a Antibody, clone APA5 validated for use in ELISA, FC, WB, IH.

Research Sub CategoryGrowth Factors & Receptors

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Purified immunoglobulin in PBS, 150 mM NaCl, pH 7.2, containing 0.09% sodium azide.

Specificity

Specifically recognizes mouse CD140a, the alpha chain of PDGF receptor. A receptor tyrosine kinase, PDGFRa forms dimers on the surface upon ligand binding and phosphorylates substrates. CD140a is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues.

Storage and Stability

Maintain at 2-8°C for up to 12 months from date of receipt.